- In 2366, a Queen, along with Locutus of Borg, an assimilated Jean-Luc Picard - led a Cube on a mission to assimilate Earth and the Federation. Fortunately, the crew of the U.S.S. Enterprise-D were able to rescue Picard and destroy the Cube, but the Queen was able to survive by unknown means.
- The Queen led a second attack on Earth in 2373, this time attempting to travel back in time and prevent the flight of the Phoenix. The Queen was defeated by the crew of the Enterprise-E.
- A second Queen, an assimilated member of Species 125, had been created by 2375. In that year she operated out of Unimatrix 01, a massive Unicomplex in the Delta Quadrant. She attempted to capture Seven of Nine from the U.S.S. Voyager, but Voyager was able to destroy her vessel.
- In 2376 a Queen attempted to crush an insurrection based out of "Unimatrix Zero", a virtual realm populated by errant Borg drones. She was foiled by Voyager the following year.
- In 2378, the Queen tried to protect a transwarp hub in the Delta Quadrant, but failed due to the actions of a future Kathryn Janeway. The Queen died, and moments later her entire Unicomplex was destroyed.
- In 2409, a new Queen has emerged and leads the Collective in a massive invasion of the Alpha and Beta Quadrants, mainly targeting the Federation and the Klingon Empire.
- “Into the Hive”: The players engage a Queen aboard her newly-constructed Unicomplex.
- “Hive Onslaught”: The Queen escaped the Unicomplex by transferring her consciousness into her vessel. She then fights Battle Group Omega, led by the U.S.S. Houston.
- “Borg Disconnected”: The Queen was revived yet again in 2410 and controls her octahedron in a hopeless battle against Voth, Undine, and Delta Alliance forces.
- “The Measure of Morality (Part 2)”: The player faces an Excalbian simulacrum of the Borg Queen; she is then slain by a simulacrum of Seven of Nine who becomes a Borg Queen herself and goes rogue, threatening to assimilate the Excalbians themselves.
- Unlike her predecessors, the Borg Queen encountered in “Into the Hive” appears to be an assimilated Romulan.
- Borg Queen at Memory Alpha, the Star Trek Wiki.
- Borg Queen at Memory Beta, the non-canon Star Trek Wiki.