- “Return to Babel”: The player saves Kirk from a Na'kuhl agent, and stabilizes his unconscious body while Doctor McCoy is en route.
- “The Measure of Morality (Part 2)”: Captain Kirk (or a simulacrum of him) commands the U.S.S. Enterprise against a rogue Excalbian Borg Queen simulacrum.
- James Kirk was the first Captain character from any of the Star Trek series to make an indirect in-game appearance in STO, he was succeeded by Gabriel Lorca.
- Kirk's dialogue in "Measure of Morality (Part 2)" is pre-recorded voice lines from the original Star Trek series.
- James T. Kirk at Memory Alpha, the Star Trek Wiki.
- James T. Kirk at Memory Beta, the non-canon Star Trek Wiki.